Labeling Reagents for HPLC

HPLC is utilized extensively as a means of detecting and determining trace components. Labeling objective substances for analysis with labeling reagents appropriate for detection methods, has been performed in order to obtain higher sensibility and selectivity. A great number of labeling reagents have been reported for this purpose. NBD- and DBD-derivatives are excellent fluorescent labeling reagents with strong long wave-length fluorescence resulting from their benzoxadiazole structure.
For electrochemical detectors ferrocene derivatives have been reported. Ferrocenes have been utilized as the most suitable labeling reagents for electrochemcial detectors because they respond to the application of relatively low voltages, such as +0.5 V, and they are excellent redox system of oxidation and reduction. N-(4-Aminobutyl)-N-ethylisoluminol has been reported to be usable as a chemiluminescence labeling reagent.

UV Detection

Fluorescence Detection

Chemiluminescence Detection

Electrochemical Detection

UV Detection

for Carboxyl Groups

B0949 B0949 B0633 B0633 A5505 A5505
M0815 M0815 A5501 A5501 A5502 A5502
A5503 A5503 A5504 A5504 A5506 A5506
A5508 A5508
B0949 1-Benzyl-3-p-tolyltriazene [for Esterification]
B0633 2-Bromo-4'-phenylacetophenone
A5505 3'-Methoxyphenacyl Bromide [for HPLC Labeling]
M0815 3'-Methoxyphenacyl Bromide
A5501 4-Bromophenacyl Bromide [for HPLC Labeling]
A5502 9-Chloromethylanthracene [for HPLC Labeling]
A5503 N-Chloromethyl-4-nitrophthalimide [for HPLC Labeling]
A5504 N-Chloromethylphthalimide [for HPLC Labeling]
A5506 O-(4-Nitrobenzyl)-N,N'-diisopropylisourea [for HPLC Labeling]
A5508 Phenacyl Bromide [for HPLC Labeling]
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for Amino Groups

N0713 N0713 N0714 N0714 I0125 I0125
I0190 I0190 A5514 A5514 A5515 A5515
A5512 A5512 A5511 A5511 D1653 D1653
M0721 M0721 N0176 N0176 F0197 F0197
B0105 B0105 D1382 D1382 A5522 A5522
A5524 A5524 D2259 D2259 A5523 A5523
I0015 I0015 A5513 A5513 S0004 S0004
N0713 (1R,2R)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0714 (1S,2S)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
I0125 1-Naphthyl Isocyanate
I0190 1-Naphthyl Isothiocyanate
A5514 2,3,4,6-Tetra-O-acetyl-beta-D-glucopyranosyl Isothiocyanate [for HPLC Labeling]
A5515 2,3,4,6-Tetra-O-benzoyl-beta-D-glucopyranosyl Isothiocyanate [for HPLC Labeling]
A5512 2,4-Dinitrofluorobenzene [for HPLC Labeling]
A5511 3,5-Dinitrobenzoyl Chloride [for HPLC Labeling]
D1653 4-(Dimethylamino)azobenzene 4'-Isothiocyanate
M0721 4-Methoxybenzoyl Chloride
N0176 4-Nitrobenzoyl Chloride
F0197 9-Fluorenylmethyl Chloroformate [N-Protecting Agent for Peptides Research]
B0105 Benzoyl Chloride
D1382 Dabsyl Chloride [N-Protecting Agent for Peptides Research]
A5522 N-Succinimidyl 4-Nitrophenylacetate [for HPLC Labeling]
A5524 Nalpha-(5-Fluoro-2,4-dinitrophenyl)-D-leucinamide [HPLC Labeling Reagent for e.e. Determination]
D2259 Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-alaninamide [HPLC Labeling Reagent for e.e. Determination]
A5523 Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide [HPLC Labeling Reagent for e.e. Determination]
I0015 Ninhydrin
A5513 Phenyl Isothiocyanate [for HPLC Labeling]
S0004 Salicylaldehyde
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for Hydroxyl Groups

N0713 N0713 N0714 N0714 A5511 A5511
M0721 M0721 N0176 N0176 B0105 B0105
N0713 (1R,2R)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0714 (1S,2S)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A5511 3,5-Dinitrobenzoyl Chloride [for HPLC Labeling]
M0721 4-Methoxybenzoyl Chloride
N0176 4-Nitrobenzoyl Chloride
B0105 Benzoyl Chloride
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for Carbonyl Groups

D0845 D0845 A5531 A5531 A5533 A5533
A5532 A5532
D0845 2,4-Dinitrophenylhydrazine (wetted with ca. 50% Water, containing 25g and 500g on a dry weight basis respectively)
A5531 2,4-Dinitrophenylhydrazine Hydrochloride [for HPLC Labeling]
A5533 Dabsyl Hydrazine [for HPLC Labeling]
A5532 O-4-Nitrobenzylhydroxylamine Hydrochloride [for HPLC Labeling]
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Fluorescence Detection

for Carboxyl Groups

A5561 A5561 A5563 A5563 A5560 A5560
A5562 A5562 A5553 A5553 A5570 A5570
A0979 A0979 B1926 B1926 A5502 A5502
A5576 A5576 A5551 A5551 A5571 A5571
A5574 A5574 A5555 A5555 A5573 A5573
A5554 A5554
A5561 (R)-(-)-DBD-APy [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5563 (R)-(-)-NBD-APy [=(R)-(-)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5560 (S)-(+)-DBD-APy [=(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5562 (S)-(+)-NBD-APy [=(S)-(+)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5553 3-Bromomethyl-7-methoxy-1,4-benzoxazin-2-one [for HPLC Labeling]
A5570 4-Bromomethyl-6,7-dimethoxycoumarin [for HPLC Labeling]
A0979 7-Acetoxy-4-bromomethylcoumarin [for HPLC Labeling]
B1926 9-(Bromomethyl)acridine [for HPLC Labeling]
A5502 9-Chloromethylanthracene [for HPLC Labeling]
A5576 AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]
A5551 Br-Mmc (=4-Bromomethyl-7-methoxycoumarin) [for HPLC Labeling]
A5571 DBD-CO-Hz [=4-(N,N-Dimethylaminosulfonyl)-7-(N-hydrazinocarbonylmethyl-N-methyl)amino-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5574 DBD-ED [=4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5555 DBD-PZ [=4-(N,N-Dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5573 NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5554 NBD-PZ (=4-Nitro-7-piperazino-2,1,3-benzoxadiazole) [for HPLC Labeling]
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for Amino Groups

A1657 A1657 N0713 N0713 A1658 A1658
N0714 N0714 A5565 A5565 A5566 A5566
A5568 A5568 A5577 A5577 A5569 A5569
A5578 A5578 A5564 A5564 A5567 A5567
A5594 A5594 M0722 M0722 A5579 A5579
D3178 D3178 F0197 F0197 D0005 D0005
D0656 D0656 A5558 A5558 A5595 A5595
A5575 A5575 F0192 F0192 F0026 F0026
A5592 A5592 A5572 A5572 A5593 A5593
P0280 P0280 S0503 S0503
A1657 (1R,2R)-2-(Anthracene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0713 (1R,2R)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A1658 (1S,2S)-2-(Anthracene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0714 (1S,2S)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A5565 (R)-(+)-DBD-Pro-COCl [=(R)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5566 (R)-(+)-NBD-Pro-COCl [=(R)-(+)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5568 (R)-(-)-DBD-Py-NCS [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5577 (R)-(-)-NBD-Py-NCS [=(R)-(-)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5569 (S)-(+)-DBD-Py-NCS [=(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5578 (S)-(+)-NBD-Py-NCS [=(S)-(+)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5564 (S)-(-)-DBD-Pro-COCl [=(S)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5567 (S)-(-)-NBD-Pro-COCl [=(S)-(-)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5594 2,3-Naphthalenedialdehyde [Fluorimetric reagent for primary Amines]
M0722 2-Methoxy-2,4-diphenyl-3(2H)-furanone [for HPLC Labeling]
A5579 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl Chloride Hydrochloride [for HPLC Labeling]
D3178 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoic Acid
F0197 9-Fluorenylmethyl Chloroformate [N-Protecting Agent for Peptides Research]
D0005 Dansyl Chloride (10% in Acetone)
D0656 Dansyl Chloride
A5558 DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-Chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5595 DBD-F [=4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5575 DBD-NCS [=4-(N,N-Dimethylaminosulfonyl)-7-isothiocyanato-2,1,3-benzoxadiazole] [for HPLC Labeling and Edman Degradation]
F0192 Fluorescamine [for HPLC Labeling]
F0026 Fluorescein Isothiocyanate (isomer I)
A5592 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
A5572 NBD-COCl [=4-(N-Chloroformylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5593 NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
P0280 o-Phthalaldehyde [for HPLC Labeling]
S0503 Succinimidyl 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoate
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for Hydroxyl Groups

A1657 A1657 N0713 N0713 A1658 A1658
N0714 N0714 A5565 A5565 A5566 A5566
A5564 A5564 A5567 A5567 A0411 A0411
A5579 A5579 D3178 D3178 D0005 D0005
D0656 D0656 A5558 A5558 A5572 A5572
A1657 (1R,2R)-2-(Anthracene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0713 (1R,2R)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A1658 (1S,2S)-2-(Anthracene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0714 (1S,2S)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A5565 (R)-(+)-DBD-Pro-COCl [=(R)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5566 (R)-(+)-NBD-Pro-COCl [=(R)-(+)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5564 (S)-(-)-DBD-Pro-COCl [=(S)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5567 (S)-(-)-NBD-Pro-COCl [=(S)-(-)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A0411 2-Aminopyridine
A5579 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl Chloride Hydrochloride [for HPLC Labeling]
D3178 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoic Acid
D0005 Dansyl Chloride (10% in Acetone)
D0656 Dansyl Chloride
A5558 DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-Chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5572 NBD-COCl [=4-(N-Chloroformylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
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for Carbonyl Groups

A5581 A5581 A0267 A0267 A5552 A5552
A5571 A5571 A5556 A5556 A5573 A5573
A5557 A5557
A5581 1,3-Cyclohexanedione [for HPLC Labeling]
A0267 2-Aminobenzenethiol
A5552 Dansyl Hydrazine [for HPLC Labeling]
A5571 DBD-CO-Hz [=4-(N,N-Dimethylaminosulfonyl)-7-(N-hydrazinocarbonylmethyl-N-methyl)amino-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5556 DBD-H [=4-(N,N-Dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5573 NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5557 NBD-H (=4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) [for HPLC Labeling]
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for Mercapto Groups

A5597 A5597 A5596 A5596 A5595 A5595
P1214 P1214 A5591 A5591 A5592 A5592
A5593 A5593
A5597 ABD-F [=4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for Determination of Thiols]
A5596 DAABD-Cl [=4-[2-(Dimethylamino)ethylaminosulfonyl]-7-chloro-2,1,3-benzoxadiazole] [for Proteome Analysis]
A5595 DBD-F [=4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling]
P1214 N-(1-Pyrenyl)maleimide
A5591 NAM [=N-(9-Acridinyl)maleimide] [for HPLC Labeling]
A5592 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
A5593 NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
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Chemiluminescence Detection

D2339 D2339 A5304 A5304
D2339 2,3-Dihydro-6-isothiocyanato-1,4-phthalazinedione [HPLC Labeling Reagent for Chemiluminescent]
A5304 N-(4-Aminobutyl)-N-ethylisoluminol [Chemiluminescence Reagent]
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Electrochemical Detection

H0941 H0941 F0406 F0406 F0280 F0280
S0599 S0599
H0941 (Hydrazinocarbonyl)ferrocene [for HPLC Labeling]
F0406 Ferroceneacetic Acid
F0280 Ferroceneboronic Acid (contains varying amounts of Anhydride) [Cyclic boronating reagent for GC/MS]
S0599 Succinimidyl (2R)-6-(Tetrahydro-2H-pyran-2-yloxy)-2,5,7,8-tetramethylchroman-2-carboxylate
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